Inducible deletion of gp96 reveals its critical role for expression of multiple integrins. WT or gp96 KO mice were injected with tamoxifen (TAM) intraperitoneally for 14 days followed by analysis. (A) Immunoblot for gp96 and β-actin (a loading control) from multiple organs: Li indicates liver; Lu, lung; Ki, kidney; Spl, spleen; Il, ileum; and Co, colon. (B) Flow cytometric analysis of BM Gr1⁺ cells for cell-surface expression of indicated molecules. Dotted histogram represents KO cells; solid line indicates WT cells. (C) Summary of gp96-dependent and independent integrins on the hematopoietic system. These data are based on numerous experiments.