NK-cell differentiation includes DN NK cells. (A) Representative flow cytometry analysis of the expression of CD3, EGFP, CD122, NK1.1, CD27, and CD11b by splenic lymphocytes of NDE mice showing the gates used to identify NK cells and NK-cell subsets defined by CD27/CD11b expression. (B) Frequency of DN NK cells among total NK cells from different organs of NDE mice; n = 4 to 30 mice. (C) Expression of various cell-surface or intracellular molecules by gated splenic DN NK cells versus the other NK-cell subsets. Results are representative of 3 experiments.