ChIP analysis of the Bim and FoxO3a promoter. ChIP analysis of the Bim promoter (A-C) and FoxO3a promoter (B-D) in the Karpas707 (A-B) and OPM-2 (C-D) cells after treatment with IGF-1 for 4 days. Chromatin DNA was immunoprecipitated with antibodies for normal IgG (negative control), anti–acetyl-histone H3 lysine 9 (Ac H3K9), anti–trimethyl-histone H3 lysine 4 (3M H3K4), anti–dimethyl-histone H3 lysine 9 (2M H3K9), and anti–histone H3 (positive control). A DNA fragment corresponding to the Bim or FoxO3a promoter region was amplified by quantitative RT-PCR. Each ChIP and RT-PCR were repeated, respectively, 3 and 2 times (*P < .05). SDs refer to the 3 independent experiments.