PKC-δ regulates GPVI-, but not PAR-mediated, phosphorylation of SHIP-1. Washed and indomethacin-treated murine platelets from PKC-δ^−/− and age-matched WT littermates were stimulated with (A) PAR4 agonists or (B) CVX for 2 minutes. The samples were then lysed with sample buffer containing dithiothreitol (DTT) and probed for pSHIP-1 Y1020 antibody. Total Erk and β-actin were used as lane-loading controls.