Time course of PARs and GPVI-mediated SHIP-1 phosphorylation. Washed and aspirin-treated human platelets were stimulated with 500 μM AYPGKF (A), 10 μM SFLLRN (B), or 100 ng/mL CVX (C) for various time periods at 37°C under stirring conditions, and the blots were probed with the antibody for pSHIP-1 Y1020. Each blot is a representative of 3 experiments from 3 different donors.