c-Myb and GATA-2 expression in 4-HT–treated 32D-BCR/ABL-C/EBPα-ER cells. Western blots (representative of 3 experiments) show c-Myb (A) and GATA-2 (B) expression in 4-HT–treated 32D-BCR/ABL cells expressing wild-type or mutant C/EBPα-ER. (C) RT-PCR amplification of c-Myb or GATA-2 pre-mRNA in 4-HT–treated 32D-BCR/ABL-C/EBPα-ER cells using pairs of intron 1 c-Myb– or GATA-2–specific primers. RT-PCR amplification of pre-GAPDH RNA was used as control. Representative of 4 different experiments.