Skin lesions, skin biopsy specimen, T-cell receptor repertoire, microsatellite markers, and γc expression. (A) Alopecia. (B) Generalized scaling erythroderma. (C) Hematoxylin and eosin, original magnification ×40. (D) Hematoxylin and eosin, original magnification ×100. Micrographs were acquired using an Olympus BX51 microscope (Olympus, Melville, NY) fitted with 10× eyepieces, Olympus UPlanApo 4×/0.10 numeric aperture and 10×/0.40 numeric aperture objectives, a CoolSNAP cf CCD camera (Photometrics, Tucson, AZ), and Openlab image acquisition software version 3.1 (Improvision, Waltham, MA). Images were processed in Photoshop CS2 (Adobe Systems, San Jose, CA). (E) Expression profile of TCR variable β (VB) subfamilies. Peripheral blood samples were stained with monoclonal antibodies (mAbs) for individual TCR VB together with anti-CD4 and anti-CD8 mAbs. The percentage of each TCR VB expression within CD4⁺ or CD8⁺ T cells was analyzed by FACS. Error bars represent SD. (F) CDR3 spectratyping. Each TCR VB fragment was amplified from cDNA with one of the VB-specific primers. The size distribution of PCR products was determined by an automated sequencer and GeneScan software. (G) Microsatellite analysis. Two different markers were amplified with FAM-labeled specific primers and subjected to GeneScan analysis. HO-1 indicates heme oxygenase-1. (H) Analysis of γc expression. Shown are the results of γc expression on lymphocytes and lymphocyte subsets. Solid peaks indicate control Ab; open peaks represent anti-CD132 mAb.