ONX0912-induced apoptosis is partially mediated by activation of JNK. (A) BCWM.1 cells were cultured with ONX0912 (2.5-50nM) for 12 hours. Whole-cell lysates were subjected to Western blotting using anti–p-MKK7, –p-SAP/JNK, and -actin antibodies. (B) BCWM.1 cells were cultured with ONX0912 (20nM, 50nM) in presence or absence of the JNK inhibitor SP600125 (10μM) and cytotoxicity was assessed by MTT assay. (C) BCWM.1 cells were cultured with ONX0912 (20nM, 50nM), in presence or absence of SP600125 (10μM) for 12 hours. Whole-cell lysates were subjected to Western blotting using anti–p-SAPK/JNK, -PARP, –caspase-9, –caspase-3, –caspase-8, and –β-actin. In all panels, error bars represent SD.