KLF2 inhibits nuclear localization of P-c-Jun and P-ATF2 in an actin-dependent manner. Immunofluorescent photomicrographs and quantification thereof of mock- (A, E, I, M, Q, U, C, G, K, O, S, and W) and KLF2-transduced (B, F, J, N, R, V, D, H, L, P, T, and X) HUVECs treated with DMSO (A-B, E-F, I-J, M-N, Q-R, U-V) or cytochalasin D (200nM; C-D, G-H, K-L, O-P, S-T, W-X) for 16 hours. Phosphorylated c-Jun on serine residue 63 (A-H) or serine residue 73 (I-P) or phosphorylated ATF2 on threonine residue 71 (Q-X) are stained red and nuclei are stained blue with Hoechst 33 342 (E-H, M-P, and U-X). (Y) Average nuclear intensity was measured for the indicated fluorescent staining and condition (n = 5). For each staining, 1-way analysis of variance with Bonferroni correction for multiple comparisons was performed, comparing KLF2/DMSO vs Mock/DMSO and KLF2/CytD vs Mock/CytD. ***P < .001. N.S. indicates not significant (P > .05).