KLF2 inhibits phosphorylation and nuclear localization of JNK in an actin-dependent manner. (A) Fluorescent photomicrographs of mock- and KLF2-transduced cells treated with dimethyl sulfoxide (DMSO) or 200nmol/L Cytochalasin D (CytD) for 16 hours, demonstrating filamentous actin in red and nuclei in blue. (B) Western blot analysis of P-JNK1, 2, and 3 levels in mock- (□) and KLF2-transduced HUVECs (■) cultured in the presence of dimethyl sulfoxide (control) or cytochalasin D (200nmol/L) for 16 hours. α-Tubulin was used as a loading control. Quantifications are shown for 4 independent experiments. (C) Immunofluorescent photomicrographs of mock- and KLF2-transduced HUVECs, with phosphorylated JNK (P-JNK) shown in red and nuclei in blue. *P < .05.