Time-course analysis after lentiviral KLF2 overexpression. (A) HUVECs were transduced with mock (M) or KLF2 (K) lentivirus. After 24, 48, or 72 hours or 7 days, RNA was isolated, and microarray expression profiling was performed. A modified Venn analysis of the microarray expression data at 24, 48, 72, and 168 hours after lentiviral KLF2 transduction is displayed. Indicated are the numbers of genes per time point that meet the indicated criteria but do not meet the criteria for an earlier time point. Inflammatory genes, transforming growth factor-β (TGF-β) signaling genes and vasodilatation genes were previously published to be KLF2 dependent. HUVECs were transduced with mock or KLF2 lentivirus for 24, 48, and 72 hours, and levels of the indicated genes were measured by the use of real-time RT-PCR (n = 5; B) or Western blot and densitometric quantification (n = 3; C). α-Tubulin was used as a loading control. (D) Summary of levels of the different posttranslationally activated forms of FAK in KLF2-transduced compared with mock-transduced cells, as analyzed with the Kinex antibody microarray. The category axis shows antibody specificity for the indicated phosphorylated amino acid residue. Fold induction by KLF2 is indicated on the y-axis. *P < .05, **P < .01.