Chim3-LV, but not empty-LV, confers survival advantage to transduced cells upon HIV-1 infection and precludes HIV-1 DNA integration. (A) The 60:40 mock-empty and mock-Chim3 CEMA3.01 cell mixtures were either left uninfected or infected with NL4-3 HIV-1 at an MOI of 0.01. The expression of ΔLNGFR, indicating the enrichment of transduced cells, has been evaluated by FACS for 35 days. (B) The infected 60:40 mock-empty and mock-Chim3 cell mixtures of panel A were analyzed for viral production by RT activity assay. (C) The HIV-1 DNA copy number was calculated by quantitative TaqMan PCR analysis in either mock- or LV-transduced cells that were separated using the anti-ΔLNGFR Ab from the respective mixtures after 11 days of NL4-3 HIV-1 infection at an MOI of 0.01. Results are means ± SEM of n = 9.