Quantitative chromatin immunoprecipitation (ChIP) analysis of the PU.1/Sfpi1 locus in G1ME cells untransduced, or transduced with MIGR1-GATA-1, examined at 24 hours and 48 hours after infection. The relative occupancy of GATA-2 (A), GATA-1 (B), PU.1 (C), Friend of GATA-1 (FOG-1, D), metastasis associated protein-2 (MTA-2, E), and acetylase H3 (acH3, F) are indicated as vertical bars. As a negative control, ChIP experiments were performed with isotype-matched preimmune IgG. The hypersensitivity 3 (HS3) region of the β-globin locus and GPIIb promoter region are controls. * denotes not done. The results represent the average of 3 independent ChIP experiments. Error bars represent SD.