Western blot analysis of JAK2 levels in cHL cells after transfection. (A) JAK2, JAK3, and STAT5 P levels in L-428 cells at 48 and 72 hours after transfection with 100 nM of pre-miRNA versus control cells. JAK3 was used as control for miR-135a specificity. In concordance with decreasing JAK2 levels, a slight decrease in levels of the downstream target phosphorylated STAT5 was observed. (B) Validation of the down-regulation effect produced by miR-135a on JAK2 in HDMYZ and L-1236 cHL cell lines.