Ets1 and Ets2 directly regulate antiapoptotic genes in vivo and in vitro. (A) Target gene expression in GFP-tagged embryonic endothelial cells isolated by high-speed cell sorting from double-mutant and control mice as indicated. mRNA levels of target genes were standardized to PECAM/CD31 mRNA levels. Cells from 3 individual embryos for each genotyped were analyzed in duplicate experiments. Error bars indicate standard deviation. An asterisk indicates a significant difference between control and double mutant (P < .05) as determined by Student t test. (B) ChIP experiments in cultured aortic ECs, quantified by qPCR. Anti-Ets1, anti-Ets2, and anti–Ets-pThr72 phospho-specific antibodies were used. (C) Analysis of target gene expression by qPCR in cultured aortic ECs with and without Ets1/Ets2 as indicated. ND indicates not detected.