AKT1 and AKT2 are not required for the development of myeloid precursor populations in vivo. (A) Gating scheme used to identify the GMP, CMP, and MEP populations present in the bone marrow of mice that were analyzed 16 weeks after the secondary serial bone marrow transplantation. The populations in panel A are gated on the singlet, DAPI-negative, lineage-negative population (left) and the singlet, DAPI-negative, lineage-negative, c-Kit⁺ population (right). (B) The percentage of CD45.2⁺Ly5^B6+ cells present in the LSK, CMP, GMP, and MEP populations from the mice described in panel A. LSK data are the same as the secondary transplantation in Figure 3C. The graph represents the mean ± SEM (n = 4).