Cdc42 deficiency causes decreased B-cell growth and increased apoptosis. (A) Splenic B cells were purified from WT and Cdc42−/− (KO) mice using CD43 microbeads, and cultured for 48 hours on 96-well plates (2 × 105 cells/well) with no stimulation, or with 2 μg/mL anti-IgM F(ab′)2 antibody or LPS. Cell growth rates were analyzed using a nonradioactive cell proliferation assay kit. Data were expressed as fold of growth relative to the growth rate of unstimulated cells. (B) Purified B cells were cultured for 72 hours on 96-well plates (2 × 105 cells/well) with no stimulation or with 2 μg/mL anti-IgM F(ab′)2 antibody and/or 50 ng/mL BAFF. Cells were then stained with annexin V followed by flow cytometric analysis (**P < .01; *P < .05). Data are means ± SD.
