Effect of point mutagenesis on FX binding by SPR. (A) Viewlite images of mutations in the Ad5 hexon for each virus. (B) Virus titers and VP/pfu ratios for each virus produced. (C) SPR analysis of virus interaction with immobilized FX (500 RU). Sensorgrams of 10¹¹ VP/mL injected at a flow rate of 30 μL/minute.