Cleavage of C-terminal deletion substrates. GST-VWF46, GST-VWF35, and GST-VWF28 (60-100 nM) were incubated with the indicated proteases (1 nM) for 5 hours or 24 hours without (−) or with (+) 10 mM EDTA. Substrates and 28-kDa cleavage products () were detected by gel electrophoresis and Western blotting with anti-GST antibody. The blots for GST-VWF28 were exposed to film for 35 seconds; other blots were exposed to film for 15 seconds. Results are representative of 3 independent experiments.