Confirmation of protein structure and fibril formation. (A) ALMC-1 and ALMC-2 display a β sheet conformation in the presence and absence of 0.5 M of Na₂SO₄ by Far UV-CD. (B) ALMC-1 and ALMC-2 λLC thermodynamic stability is slightly increased in the presence of 0.5 M of Na₂SO₄. (C) ALMC-1 and ALMC-2 endpoint ThT fluorescence fibril formation samples at their T_m. The excitation wavelength was 450 nm; emission wavelengths were 470 to 530 nm with a maximum fluorescence enhancement at 485 nm plotted for each sample with ThT baseline subtracted from them. Representative results from a single experiment are shown.