Expression of Akt isoforms in B-lineage cells. (A) Relative Akt isoform expression in sorted BM, splenic, and peritoneal B-cell populations measured by qRT-PCR. Data are expressed relative to wild-type follicular B cells. Brain and liver cDNA serve as positive controls for Akt3 and Akt2 mRNA, respectively. (B) Δ-Ct values for Akt1, Akt2, and Akt3 relative to endogenous control 18s, within each of the indicated B-cell populations as in panel A. (C) Akt3 transcript abundance in sorted B-cell populations from Akt1/2 DKO chimeras relative to the corresponding wild-type population. Error bars represent relative quantity maximum and minimum for each sample (A,C) or SD of Δ-Ct for each sample (B).