Akt1/2-deficient B cells compete poorly with wild-type B cells. (A) Double chimeras were analyzed at 16 weeks after transplantation. (A) Representation of the indicated B-cell subset derived from WT (Ly5^SJL+) or Akt1^+/+Akt2^+/− (top) or Akt1^−/−Akt2^−/− (bottom) progenitors. Each subset was gated as shown in Figure 2. (B) The average ratio of cells in the indicated subpopulation derived from Ly5^B6+ and Ly5^SJL+ progenitors in double chimeras established with Akt1^+/+Akt2^+/− or Akt1^−/−Akt2^−/− progenitors was calculated using gates shown in Figures 1 and 2 with 4 or 5 animals per group. Individual mice are indicated by ◇ and ×. LSK indicates Lineage⁻ c-Kit⁺ Sca-1⁺. Data are representative of 2 separate experiments.