Analysis of the VEGFR2 promoter. (A) Effect of E2-2 on 5′ deletion mutants of the VEGFR2 promoter. MEECs were transfected with 1 of the indicated reporters with different amounts of E2-2. The inhibition percentage of reporter activities by E2-2 is indicated to the right of the top panel. (B) Effect of E2-2 on 3′ deletion mutants of the VEGFR2 promoter. The experiment was carried out as described in panel A. (C) Effect of Id1 on E2-2–mediated suppression of VEGFR2 promoter activity. Different amounts of Id1 (0.1 and 0.5 pg) were transfected together with E2-2. The experiment was carried out as described in panel A.