HES1 dependence on the Fanconi anemia pathway. (A) FANCA and FANCG failed to immunoprecipitate with HES1 in FA mutant cells. Normal HSC93 and FA mutant lymphoblasts (FA-C: HSC536; FA-F: EUFA698) were subjected to immunoprecipitation with anti-HES1 antibodies. The immunoprecipitates were analyzed by Western blotting with the indicated antibodies. (B) Cell extracts from HeLa, PD430 (FA-A), and PD430 cells that were complemented with the FANCA gene (FA-A + FANCA) were subjected to immunoprecipitation using anti-FANCG or anti-HES1 antibodies and were immunoblotted with the indicated antibodies.