2D-DIGE analysis of human platelets incubated with control IgG1 (left column), or after activation of GPVI receptor (HGP4C9 mAb 1 μg/mL, right column) at different pH gradients: 4.5-6.8 (top row) and 6.25-8.6 (bottom row). Images show representative gels of proteins labeled with the fluorescent cyanine dyes Cy5 (control) and Cy3 (GPVI-activated sample). Differentially abundant proteins are framed in green: protein spots 1 to 8 (pH 4.5-6.8, top row), and protein spots 9-13 (pH 6.25-8.6, bottom row).