CD103⁺ Treg cells reduced pathogenic CD4⁺ T cells in GVHD target tissues. Twenty days after infusion of CD103⁺ Treg cells, mononuclear cells from kidneys and livers of untreated and treated recipients were stained for CD4, CD103, IL-17, IL-10, and IFN-γ. (A) The gated CD4⁺ T cells were shown in FoxP3 versus CD103. (B) The gated CD4⁺ T cells were shown in IL-17 vs CD4. (C) Gated CD4⁺ T cells were shown in IL-10 vs IFN-γ. One representative of 4 replicated experiments is shown. The means (± SE) of percentage of FoxP3⁺ cells among CD4⁺ T cells in kidney and liver of untreated and treated recipients are 12.6% (± 2.5%) versus 31.7% (± 3.6%) and 16.8% (± 2.5%) versus 26.9% (± 4.5%), respectively. The means (± SE) of percentage of IL-17⁺ cells among CD4⁺ T cells in kidney and liver of untreated and treated recipients is 3.1% (± 1.7%) versus 1.6% (± 0.2%) and13.6% (± 2.1%) versus 7.4% (± 0.7%), respectively. The mean (± SE) percentages of IL-10⁺IFN-γ⁺ cells among CD4⁺ T cells in kidney and liver of untreated and treated recipients are 15.8% (± 3.5%) versus 1.8% (± 0.4%) and 0.8% (± 0.3%) versus 0.3% (± 0.2%), respectively. The mean (± SE) percentages of IFN-γ⁺ cells among CD4⁺ T cells in kidney and liver of untreated and treated recipients are 7.9% (± 0.9%) versus 3.8% (± 0.7%) and 19.4% (± 2.8%) versus 8.1% (± 1.2%), respectively.