CINC-1 increases in the BALF of rats treated with LPS and infused with plasma from stored PRBCs or LR-PRBCs. CINC-1 levels (ng/mL) were measured (ELISA) in the BAL fluid (BALF) from rats transfused with PRBCs () or LR-PRBCs (▧) at 5% to 10% total blood volume. Rats pretreated with NS and infused with the plasma from fresh or stored PRBCs as well as LPCs, the lipids that accumulate during routine storage of PRBCs, did not evidence any significant increases in CINC-1 compared with rats pretreated with NS and infused with NS. Moreover, animals treated with LPS and then infused with NS or LPS or the plasma from fresh (D1) PRBCs and LR-PRBCs or 5% plasma from D28 PRBC or D28 LR-PRBCs also did not evidence increases in CINC-1. However, rats injected with LPS and transfused with plasma from D28 (10%) and D42 (5% and 10%) PRBCs and LR-PRBCs had a significant increase (P < .05, n = 4) in CINC-1 levels in the BALF from rats pretreated with LPS and transfused with NS (*P < .05 from LPS/NS, n = 4).