Human-derived macrophages bind VWF and FVIII. Monocytes were derived from blood and differentiated into macrophages as described in “Preparation of human macrophages.” Binding of pd-VWF (10 μg/mL) and recombinant FVIII (50 μg/mL) was allowed for 1 hour at 4°C. Cells were stained using TRITC-conjugated anti-CD16 antibodies (A,D) in combination with either FITC-conjugated anti-FVIII antibodies (B) or FITC-conjugated anti-VWF antibodies (E). Panels C and F represent merged images of panels A and B and panels D and E, respectively. Original magnification 630×.