Effect of different siRNA to PRR on the induction of TF expression by poly I:C. (A) EC were pretreated with siRNA against TLR3, PKR, RIG-I, MDA5, as well as control siRNA against GAPDH, and 48 hours later, the amount of the respective mRNA was quantified by RT-PCR. (B) After a similar transfection with siRNA, cells were stimulated with 10 μg/mL poly I:C and 25 ng/mL TNF-α for 5 hours, and the plasma clotting time was determined. *P < .05 compared with other siRNAs in the same group using ANOVA followed by Dunnett posttest.