Figure 3
Growth activity of 5 factors in 8 HMCLs. HMCLs were starved for 2 hours and cultured without growth factor (group) or with either IL-6 (200 pg/mL) or IGF-1 (100 ng/mL) or HB-EGF (1 μg/mL) or HGF (200 ng/mL) or APRIL (200 ng/mL) for 4 days in the Syn H culture medium. The cell concentration were 2 × 105 cells/mL for XG-1, XG-2, XG-6, XG-12, and XG-14 HMCLs and 105 cells/mL for XG-5, XG-7, and XG-20 HMCLs. Results are the mean luminescent signal determined in 6 replicate culture wells and are those from 1 of 3 representative experiments. The mean value was significantly different from that obtained in the control group using a Student t test (*P ≤ .05).
