Unstimulated X-CGD RPMØs produce proinflammatory cytokines and lack alternative activation markers until treated with IL-4. (A) RPMØs were plated for 24 hours, medium was changed, and 6 hours later cytokines were measured in culture supernatants as in “Cytokines.” Data represent means plus or minus SEM; N = 3 experiments. * indicates P value less than or equal to .005 compared with WT. RPMØs were plated for 2 hours and then treated either with medium alone (B) or 10 ng/mL IL-4 for 20 hours (C). Cells were analyzed by flow cytometry for alternative activation markers (CD36, MMR, and CD14) and IL-4R; histograms are representative of N = 3 experiments.