MKO mice have impaired blood neutrophil mobilization but normal neutrophil recruitment to the peritoneum in response to Listeria infection. Control (CTL) or MKO mice were infected intraperitoneally with L monocytogenes. (A) Survival was assessed in mice (n = 12 per group) from 2 separate infections with 9.8 to 11.2 × 105 CFU of bacteria. (B) The bacterial titer in the spleen and liver of control and MKO mice (n = 8 per group) was determined 72 hours after infection with 2.1 to 7.2 × 105 CFU of bacteria. (C) Blood neutrophil counts were assessed by flow cytometry at the indicated times after infection with 2.1 to 9.8 × 105 CFU of bacteria (n = 8-19 mice per group depending on the time). (D) Shown is the number of neutrophils in the peritoneum at the indicated times after infection with 2.1 to 7.2 × 105 CFU of bacteria (n = 5-8 mice per group depending on the time). Data represent the mean ± SEM. †P < .05 compared with time 0.
