Basal granulopoiesis in MKO mice is characterized by a shift of neutrophils from the bone marrow to the blood. (A,B) The number of mature neutrophils (Gr-1^brightSSC^hi) in the blood and bone marrow was quantified by flow cytometry in mice of the indicated genotype. (C) The neutrophil distribution index (NDI) was calculated to estimate the percentage of total body neutrophils in the blood, using the following formula: NDI = blood neutrophils/(blood + bone marrow neutrophils). Data represent the mean ± SEM. #P < .05 compared with all other groups.