Genscan analysis of PCR products. Amplifications of immunoglobulin heavy chain CDR3 sequences were performed using the 3 FR1, FR2, and FR3 primer sets. PCR products were run on a sequence analyzer and sizes were determined using a size marker (in red) and the Genscan software. The figure shows representative data in patient 1 of (A) the dominant clone detected in the lung, (B) the PCR product alignment of lung and lymph node dominant clone, and (C) the comparison of lung clone and polyclonal blood B-cell repertoire.