DM induces the phosphorylation of p59Fyn and Zap70. Primary human T cells and Jurkat cells were treated with CXCL12 (100 ng/mL) and DM (1 μM) or the combination for 5 minutes and then lysed with RIPA buffer as mentioned in Figure 1. Total Fyn59 (A) and Zap70 (B) were immunoprecipitated using the total Fyn59 and Zap70 antibodies, and then phosphorylation status was assessed by immunoblotting using the antiphosphotyrosine antibody. The membrane was then reprobed using the total antibody of Fyn59 or Zap70. The densitometric value of each phospho-band was first normalized against the total (P/T) of each molecule and then presented as fold change over control.