CD133 and CD45 expression in CD14⁺ cells alone or treated with PTN, M-CSF, or the combination of both cytokines for 7 days. (A) RT-PCR analysis of CD133 and CD45 gene expression in CD14⁺ cells alone or treated with PTN, M-CSF, or the combination of these cytokines. (B) Flow cytometric analysis of CD45 expression on CD14⁺ cells following treatment with no treatment, PTN, M-CSF, or the combination of both cytokines. The proportion of cells expressing CD45 is shown. Each experiment was performed in triplicate and error bars represent multiple assays.