Analysis of VSMC-specific TF-deficient mice. (A) Genomic DNA was isolated from the tails of TF^flox/flox mice (lane 1), TF^flox/flox/SM22αCre^+/− mice (lane 2); and TF^flox/+/SM22αCre^+/− mice (lane 3). PCR was performed with primers that identify the wild-type and floxed TF alleles and the Cre recombinase. (B) mRNA was isolated from brain, lung, and kidney of TF^flox/flox (F) and TF^flox/flox/SM22αCre^+/− (C) mice and analyzed by RT-PCR for TF. GAPDH was used as an internal control for equal loading.