Representative images of endothelial VCAM-1 expression among the different treatment groups. The red fluorescent staining (655 nm Qdot bioconjugate) indicates surface VCAM-1 immunoreactivity, whereas blue staining represents nuclear Hoechst staining. (A) A2^+/+ or A2^−/− mice were treated with either IgG-APS or IgG-NHS, and LPS or PBS were used as signal controls. (B) A2^+/+ or A2^−/− mice were treated with 4C5, anti-A2 MoAb, or MuMoAbC as control, and LPS or PBS were used as signal controls.