Haploinsufficiency of PSF1 for hematopoiesis. (A,B) Cells of KSL populations in the BM from 8-week-old mice (A) and 1-year-old mice (B) were analyzed. Percentage of each fraction indicated by box was represented. R1 and R2 in panel B indicates fraction from c-kit⁺Sca-1⁻Lin⁻ cells and c-kit⁺Sca-1⁺Lin⁻ cells, respectively. (C) Quantitative evaluation in percentage of each fraction among all BM cells of 1-year-old wild-type (Wt) or PSF1^+/− (+/−) mice as indicated. Mac-1/Gr-1 (myeloid), B220 (B cells), CD4/CD8 (T cells), or TER119 (erythroid). HSCs populations were studied in a CD34⁻ KSL cell population. Populations of KSL (c-kit⁺Sca-1⁺Lin⁻ cells) and KL (c-kit⁺Sca-1⁻Lin⁻ cells) were also evaluated. *P < .05.