Expression of ZNF423 in BCR/ABL-positive cell lines and in CML BC samples. (A) mRNA (3 μg) extracted from 6 p210BCR/ABL-positive, 4 p190BCR/ABL-positive, and 3 Ph-negative cell lines was blotted with a part of the human ZNF423 coding region. β-Actin hybridization was performed as an internal control. The position of the ZNF423 message is indicated by → and the immunophenotypes of the cell lines are shown at the bottom. A vertical line has been inserted to indicate a repositioned gel lane. (B) Total RNAs extracted BM samples from 3 CML CP and 8 CML BC cases (4 myeloid and 4 B-lymphoid) were subjected to RT-PCR for ZNF423 expression. β-Actin RT-PCR was performed as an internal control.