Survival curves and pathologic and flow cytometric analyses of leukemic mice. (A) Survival curves of the mice. The survival curves of WT/BXH2 and p210BCR/ABL/BXH2 are shown by thin and thick continuous lines, respectively, whereas those of BXH2-nonbackcrossed WT and p210BCR/ABL are shown by thin and thick dotted lines, respectively. As for the 6 p210BCR/ABL/BXH2 animals that died in a short latency and exhibited nonmyeloid phenotypes (nos. 30, 1, 17, 5, 18, and 23), the death points are indicated by → and the immunophenotypes of the disease are shown in the parentheses. T indicates T-cell leukemia; and B, B-cell leukemia. (B) Pathologic analysis of the leukemic mice. WG-stained peripheral blood smears (PB) and HE-stained lymph node (LN) and liver slices of a representative mouse for B-cell leukemia (no. 17) or T-cell leukemia (no. 5) are shown. PB smears show proliferation of blast cells and LN specimen shows the destruction of the basal structure by blast cell infiltration. In the liver, blast cells are observed around the vessel and in the sinusoids. (C) Flow cytometric analysis of mice that developed B-cell or T-cell leukemia. Blast cells of no. 17 were positive for CD19 but negative for Thy1.2, Mac-1, and Gr-1 and those of no. 5 were positive for Thy1.2 but negative for CD19, Mac-1, and Gr-1, indicating that they were of B- and T-lymphoid origins, respectively. The percentages of positive cells in each quadrant are shown.