Quantitative RT-PCR validation of 8 differentially expressed genes in CD4⁺CD7⁻ Sezary cells from SS patients. The expression of several genes identified in the microarray comparing AHI-1–suppressed Hut 78/sh4 cells with Hut 78 control cells were evaluated by quantitative RT-PCR in both CD4⁺CD7⁻ primary leukemic cells from 6 SS patients and 5 CD4⁺ normal controls. (A) Validation of up-regulated genes in primary SS samples compared with normal controls: AHI-1 and PALM2-AKAP2. (B) Validation of down-regulated genes in primary SS samples compared with normal controls: HCK, BIN1, NKG7, REPS2, SPIB, and BRDG1. Values shown are the mean ± SEM. The P values presented are the result of 2-sample t tests comparing the SS patient expression data with that of normal controls.