Figure 5
![Figure 5. Growth factors and coculture with BMSCs do not protect against tunicamycin-induced BCWM.1 cell cytotoxicity. (A) BCWM.1 cells were cultured with control media, and with tunicamycin (0.5 μg/mL to 1 μg/mL) for 48 hours, in the presence or absence of BMSCs. Cell proliferation was assessed using the [3H]-thymidine uptake assay. (B) BCWM.1 cells were cultured with control media in the presence or absence of IL-6 (25 ng/mL), CD40L (3 ng/mL), or BLyS (100 ng/mL), and treated with tunicamycin (0.1 μg/mL and 1 μg/mL) for 48 hours. Cytotoxicity was assessed by the MTT assay. All data represent mean plus or minus SD of triplicate experiments (A,B).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/3/10.1182_blood-2007-10-116848/5/zh80040930010005.jpeg?Expires=1730500653&Signature=nhXWryfNF6QGZArA31Wi3H7gi6eVSWUB7MOkQ95o4B95NJ0a19mV2iaCIn3hgNZhTUAMBbH55mM56CSM6sN6h4KlfQ-llDSZeIf3xgGHEaaUzFYVESeC~r5RjXkJeuoyt-zWUoK7avkIW5fUHS~WFYdSOUhabkGm~M08tYH03beg9Po8iaWQEyZC8~a9JZ46t4l3SfXnDKYZFHZ3-d5JCVRPbnfay4B35ry1nN9kaXuhD-urQYY1B5Po73U6U9PwKScXdwWfqJB1CmgctodFgeaDti10luGbrioM5o32GOntDOY24PhGQ~pAJwtfvO1v2Vpfb~JlP7IDgS2C1Ntx1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Growth factors and coculture with BMSCs do not protect against tunicamycin-induced BCWM.1 cell cytotoxicity. (A) BCWM.1 cells were cultured with control media, and with tunicamycin (0.5 μg/mL to 1 μg/mL) for 48 hours, in the presence or absence of BMSCs. Cell proliferation was assessed using the [3H]-thymidine uptake assay. (B) BCWM.1 cells were cultured with control media in the presence or absence of IL-6 (25 ng/mL), CD40L (3 ng/mL), or BLyS (100 ng/mL), and treated with tunicamycin (0.1 μg/mL and 1 μg/mL) for 48 hours. Cytotoxicity was assessed by the MTT assay. All data represent mean plus or minus SD of triplicate experiments (A,B).
