Figure 6
Figure 6. Ectopic expression of spi-1l mRNA is sufficient to rescue myeloid markers in cloche mutants. (A-F) Lateral view of the yolk (YK) region of embryos from a heterozygous cloche intercross at 36 hpf. Probes for genes, embryo genotype, and mRNA injected are as indicated. Red arrows indicate mRNA expression in the YK region of a wild-type sibling from a cloche intercross. Wild-type sibling of cloche mutants express mpx (A) and lcp1 (B). Cloche mutant embryos lack expression of mpx (C) and lcp1 (D). Cloche embryos injected with spi-1l mRNA partially recover mpx (E) and lcp1 (F) expression. (G) Table demonstrating percentage and number of cells per embryo of cloche wild-type sibling, cloche mutant, and cloche mutant embryos injected with spi-1l mRNA, expressing mpx and lcp1.

Ectopic expression of spi-1l mRNA is sufficient to rescue myeloid markers in cloche mutants. (A-F) Lateral view of the yolk (YK) region of embryos from a heterozygous cloche intercross at 36 hpf. Probes for genes, embryo genotype, and mRNA injected are as indicated. Red arrows indicate mRNA expression in the YK region of a wild-type sibling from a cloche intercross. Wild-type sibling of cloche mutants express mpx (A) and lcp1 (B). Cloche mutant embryos lack expression of mpx (C) and lcp1 (D). Cloche embryos injected with spi-1l mRNA partially recover mpx (E) and lcp1 (F) expression. (G) Table demonstrating percentage and number of cells per embryo of cloche wild-type sibling, cloche mutant, and cloche mutant embryos injected with spi-1l mRNA, expressing mpx and lcp1.

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