Inhibition of survival signals PI3K/Akt or NFκB potentiates apoptosis. KG1a cells, cultured without stromal support, were pretreated with either wortmannin (50 nM) or IKK-2 inhibitor (Inh) IV (300 nM) for 30 minutes and cultured for 18 hours in the presence or absence of TNF-α (100 ng/mL). Apoptosis was assessed as described for Figure 1. Blockade of either pathway resulted in increased apoptosis.