Alloantigen expression on pDCs alone is sufficient to stimulate alloreactive CD8⁺ T cells. CD8⁺ T cells from AKR (CD90.2⁻) mice were transferred to irradiated β2m^−/− C3H (CD90.2⁺) mice preinjected with WT B cells, pDCs, or cDCs. Expansion of CD90.2⁻ donor CD8⁺ T cells in mLNs (A) and IFN-γ expression on donor CD8⁺ T cells in spleens (B) 6 days after transfer are shown as means plus or minus SEM. Data are representative of 2 similar experiments. *P < .05.