AONs enter the cells with high efficiency on nucleofection (NF). FACS analysis of 10⁶ activated T cells from a normal control after NF with a FAM-labeled AON. Two gates, M1 and M2, were set to determine transfection efficiency (%) and median fluorescence of the cells. (A) No AON added, with nucleofection. (B) FAM-labeled AON added, without nucleofection. (C) FAM-labeled AON added with nucleofection. NR indicates not relevant.