Figure 2
IL-12Rβ1 lacking exon 2 is a functional protein. Response to IL-12 or IL-23 stimulation of IL-12Rβ1 constructs of activated T cells from an IL-12Rβ1−/− patient. Because IL-23R expression is normally low in PHA-activated T cells, an IL23R construct was transfected as well to allow detection of IL-23 responses. Cells were prestimulated for 18 hours with anti-CD2/CD28. (A) STAT4 phosphorylation 60 minutes after stimulation. (B) STAT3 phosphorylation 15 minutes after stimulation. (C) STAT1 phosphorylation 15 minutes after stimulation. (D) IFN-γ production measured after 72 hours of stimulation of 105 cells with various concentrations of IL-12 and IL-23 in the presence of anti-CD2/CD28.
