Complement-regulated Fas and Bcl-2 involves PI-3Kγ. (A) Mean fluorescent intensities for T-cell surface Fas expression on WT (top) or C5aR^−/− (bottom) B6 T cells stimulated with anti-CD3/CD28 plus or minus recombinant C5a plus or minus 0.1 μM AS25242 (72 hours). *P < .05 versus control. The experiment was repeated with identical results. (B) Flow cytometry plot demonstrating Bcl-2 expression in anti-CD3/CD28-stimulated B6 T cells (gated on CD4⁺ cells) plus or minus 0.1 μM AS252424. Numbers in each panel depict the mean fluorescence intensity. Bcl-2 MFI in unstimulated cells was 130. Assay was performed at 72 hours. Data are representative of at least 2 independent experiments. P < .05.