Efficient CNTO 530 lessening of the anemia induced by 5-fluorouracil or paclitaxel plus carboplatin. (A) Anemia was induced via single 5-FU injection (150 mg/kg). On day 4.5, CNTO 530 (0.3 μg/mouse gram-weight), darbepoietin-alfa (0.02 μg/mouse gram-weight), or saline then was administered. Hematocrits were determined at the indicated time points. Results for 2 independent experiments are shown (means ± SE, n = 4 for each experiment). **P ≤ .001; *P ≤ .024. (B) In Sprague-Dawley rats, anemia was induced via dosing on days 1, 8, and 15 with 10 mg/kg paclitaxel (pac) + 50 mg/kg carboplatin (carbo). On day 16, CNTO 530 or darbepoietin-alfa then was administered and effects on hemoglobin levels and hematocrits were determined over a 50-day interval. Dosing for CNTO 530 was at 1 μg and 3 μg/rat gram-weight, and for darbepoietin-alfa at 0.03 μg and 0.3 μg/rat gram-weight. Mean values ± SD are graphed (n = 8 for controls, for pac/carbo, for pac/carbo + CNTO 530, and for pac/carbo + darbepoietin-alfa). The untreated group is indicated as NT (not treated). Top and bottom panels illustrate representative effects on hemoglobin levels and hematocrits.